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fgf8 100-25a  (PeproTech)


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    Structured Review

    PeproTech fgf8 100-25a
    Fgf8 100 25a, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgf8+100-25a/fgf8+growth+factor/pm40370197-108-24-26
    Average 90 stars, based on 1 article reviews
    fgf8 100-25a - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    RNA Sequencing:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Sequencing:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Protein-Protein interactions:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Fluorescence:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Over Expression:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Staining:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Whisker Assay:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Generated:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Expressing:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Western Blot:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Knockdown:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Two Tailed Test:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Gene Expression:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.

    Encapsulation:

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
    Article Snippet: The culture medium was then replaced with DMEM containing 2% FBS to starve chondrocytes for 12 h. Next, chondrocytes were treated with FGF8 (100-25A, PeproTech, 100-25, USA) at 5, 10, or 25 ng/ml in the presence or absence of SB203580 at 20 μM (MCE, HY-10256, USA) in 1% FBS DMEM.

    Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes.
    Article Snippet: The culture medium was then replaced by DMEM containing 2% FBS to starve the chondrocytes for 12 h. Next, the chondrocytes were treated with FGF8 (100-25A; Peprotech, Rocky Hill, USA) at 5, 10, or 25 ng/mL in the presence or absence of SB203580 (HY-10256; MCE, Monmouth Junction, USA) at 20 μM in 1% FBS DMEM.



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    Progressive loss of the apical ectodermal coincides with the apparition of cell death in the anterior margin of the limb. In situ hybridization of <t>Fgf8</t> gene expression and cell death pattern evidenced by lysotracker stain at limb development stages from 22 to 25 HH. Notice that cell death occurs in regions in which Fgf8 expression is disappearing.
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    Image Search Results


    Progressive loss of the apical ectodermal coincides with the apparition of cell death in the anterior margin of the limb. In situ hybridization of Fgf8 gene expression and cell death pattern evidenced by lysotracker stain at limb development stages from 22 to 25 HH. Notice that cell death occurs in regions in which Fgf8 expression is disappearing.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: Progressive loss of the apical ectodermal coincides with the apparition of cell death in the anterior margin of the limb. In situ hybridization of Fgf8 gene expression and cell death pattern evidenced by lysotracker stain at limb development stages from 22 to 25 HH. Notice that cell death occurs in regions in which Fgf8 expression is disappearing.

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: In Situ Hybridization, Expressing, Staining

    Control of PCD in the ANZ by FGF, WNT, and BMP signaling. The cell death pattern was evaluated in the ANZ at limb developing stage 24 HH after FGF, WNT, and BMP signaling treatments. (A) FGF8 treatment for 6 h did not modify the cell death pattern but inhibiting FGF signaling resulted in the promotion of PCD. (B) WNT3A treatment for 8 h did not alter the cell death pattern, but DKK-treatment to inhibit Wnt signaling induced cell death after 8 h. (C) BMP4- or BMP7- treatment for 6 h promoted cell death, whereas NOGGIN inhibited cell death after 6 h of treatment. Notice that the minimum time to trigger cell death in the ANZ is 6 h after inhibiting FGF or activating BMP signaling, whereas DKK needed 8 h to promote cell death. The images at the top of each line correspond to samples stained with Neutral Red, while the images at the bottom are stained with Lysotracker. The axis showed in A can be used for B and C.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: Control of PCD in the ANZ by FGF, WNT, and BMP signaling. The cell death pattern was evaluated in the ANZ at limb developing stage 24 HH after FGF, WNT, and BMP signaling treatments. (A) FGF8 treatment for 6 h did not modify the cell death pattern but inhibiting FGF signaling resulted in the promotion of PCD. (B) WNT3A treatment for 8 h did not alter the cell death pattern, but DKK-treatment to inhibit Wnt signaling induced cell death after 8 h. (C) BMP4- or BMP7- treatment for 6 h promoted cell death, whereas NOGGIN inhibited cell death after 6 h of treatment. Notice that the minimum time to trigger cell death in the ANZ is 6 h after inhibiting FGF or activating BMP signaling, whereas DKK needed 8 h to promote cell death. The images at the top of each line correspond to samples stained with Neutral Red, while the images at the bottom are stained with Lysotracker. The axis showed in A can be used for B and C.

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: Staining

    Inhibition of Dkk and Bmp4 gene expression by treatment with an inhibitor on FGF signaling. Gene expression pattern of Bmp4 and Dkk in the anterior margin of the limb at developing stage 24HH. (A) Dkk expression inhibition was observed after 2 h of treatment with SU5402, while Bmp4 was inhibited after 4 h. The gene expression pattern of Dkk and Bmp4 was not modified at least 4 h after (B) FGF8- and (C) FGF10-treatment.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: Inhibition of Dkk and Bmp4 gene expression by treatment with an inhibitor on FGF signaling. Gene expression pattern of Bmp4 and Dkk in the anterior margin of the limb at developing stage 24HH. (A) Dkk expression inhibition was observed after 2 h of treatment with SU5402, while Bmp4 was inhibited after 4 h. The gene expression pattern of Dkk and Bmp4 was not modified at least 4 h after (B) FGF8- and (C) FGF10-treatment.

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: Inhibition, Expressing, Modification

    BMP signaling regulates Fgf8, Bmp4, Dkk, Msx2, and Bambi gene expression. Regulation of (A–C) Fgf8 , (D–F) Bmp4, (G–I) Dkk, (J–L) Msx2, (M–O) Bambi gene expression in the anterior limb margin after BMP4, BMP7, or Noggin. Fgf8 (B, C) and Bmp4 (D, E) are downregulated after 4 h of BMP4 or BMP7. Notice the stronger effect of BMP4 than BMP7. In contrast, NOGGIN-treatment maintained the expression of both genes after treatments. (G–I) Upregulation of Dkk gene expression after 4 h of BMP4- or BMP7-treatment and downregulation of Dkk after 4 h of NOGGIN treatment. (J–L) Msx2 and (M–O) Bambi gene expression were upregulated by BMP4 and BMP7. In contrast, NOGGIN inhibited Bambi expression during the first 4 h of treatment but not Msx2 [Panel (L, O) ].

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: BMP signaling regulates Fgf8, Bmp4, Dkk, Msx2, and Bambi gene expression. Regulation of (A–C) Fgf8 , (D–F) Bmp4, (G–I) Dkk, (J–L) Msx2, (M–O) Bambi gene expression in the anterior limb margin after BMP4, BMP7, or Noggin. Fgf8 (B, C) and Bmp4 (D, E) are downregulated after 4 h of BMP4 or BMP7. Notice the stronger effect of BMP4 than BMP7. In contrast, NOGGIN-treatment maintained the expression of both genes after treatments. (G–I) Upregulation of Dkk gene expression after 4 h of BMP4- or BMP7-treatment and downregulation of Dkk after 4 h of NOGGIN treatment. (J–L) Msx2 and (M–O) Bambi gene expression were upregulated by BMP4 and BMP7. In contrast, NOGGIN inhibited Bambi expression during the first 4 h of treatment but not Msx2 [Panel (L, O) ].

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: Expressing

    The last step to induce cell death is the inhibition of FGF signaling.Double treatments were done to promote or block FGF and BMP signaling for 6 h. Under these conditions, lysotracker staining and Dkk gene expression were evaluated. (A) Double FGF8 and BMP4 treatment promoted Dkk expression, and cell death was observed closer to the BMP bead. (B) Inhibiting FGF signaling in the presence of BMP4, Dkk was still expressed. (C) Treatment with NOGGIN and in the presence of FGF8 significantly inhibited Dkk expression, but it slightly diminished the area of cell death. (D) Double treatment inhibiting FGF and BMP signaling inhibited Dkk gene expression, but no cell death. (E) Fgf8 gene expression is inhibited in response to DKK protein. Notice that the blue line of the right limb (DKK treatment)—representing the expression of Fgf8 from the limb posterior margin to the anterior limb margin—is shorter than the contralateral limb.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: The last step to induce cell death is the inhibition of FGF signaling.Double treatments were done to promote or block FGF and BMP signaling for 6 h. Under these conditions, lysotracker staining and Dkk gene expression were evaluated. (A) Double FGF8 and BMP4 treatment promoted Dkk expression, and cell death was observed closer to the BMP bead. (B) Inhibiting FGF signaling in the presence of BMP4, Dkk was still expressed. (C) Treatment with NOGGIN and in the presence of FGF8 significantly inhibited Dkk expression, but it slightly diminished the area of cell death. (D) Double treatment inhibiting FGF and BMP signaling inhibited Dkk gene expression, but no cell death. (E) Fgf8 gene expression is inhibited in response to DKK protein. Notice that the blue line of the right limb (DKK treatment)—representing the expression of Fgf8 from the limb posterior margin to the anterior limb margin—is shorter than the contralateral limb.

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: Inhibition, Blocking Assay, Staining, Expressing

    Establishment of the ANZ by the WNT-BMP-FGF regulatory network. In this model, WNT3A and BMP signaling antagonistically regulate Fgf8 expression and consequently cell death. WNT3A induces the expression of Fgf8 in the AER. BMP signaling inhibited Fgf8 expression; meanwhile, DKK inhibits the function of WNT/ß catenin signaling, and consequently, the expression of Fgf8 is inhibited. In the presumptive ANZ, the high levels of FGF8 from AER are necessary for cell survival, but FGF8 is also required for promoting cell death regulating Bmp4 and Dkk expression. The establishment of ANZ occurs when the levels of FGF signaling are reduced and BMP signaling increases leading to the inhibition of Fgf8 in the AER inducing cell death. Fgfr1, Fgfr2, Mkp3, Churchill, Msx2, Id2, and Bambi are expressed differentially in the presumptive ANZ and the established ANZ.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

    doi: 10.3389/fcell.2021.703836

    Figure Lengend Snippet: Establishment of the ANZ by the WNT-BMP-FGF regulatory network. In this model, WNT3A and BMP signaling antagonistically regulate Fgf8 expression and consequently cell death. WNT3A induces the expression of Fgf8 in the AER. BMP signaling inhibited Fgf8 expression; meanwhile, DKK inhibits the function of WNT/ß catenin signaling, and consequently, the expression of Fgf8 is inhibited. In the presumptive ANZ, the high levels of FGF8 from AER are necessary for cell survival, but FGF8 is also required for promoting cell death regulating Bmp4 and Dkk expression. The establishment of ANZ occurs when the levels of FGF signaling are reduced and BMP signaling increases leading to the inhibition of Fgf8 in the AER inducing cell death. Fgfr1, Fgfr2, Mkp3, Churchill, Msx2, Id2, and Bambi are expressed differentially in the presumptive ANZ and the established ANZ.

    Article Snippet: Heparin beads (Cat. H6508, Sigma-Aldrich, St. Louis, MO, United States) or in Affigel (Bio-Rad Laboratories, Hercules, CA) were soaked in 1 mg/ml FGF8 (cat. 100-25A), FGF10 (100-26), 1 mg/ml DKK (cat. 120-30) BMP4 (cat. 120-05), BMP7 (cat. 120-30P) or 2 mg/ml NOGGIN (cat. 120-10C) from Peprotech, Mexico City, Mexico.

    Techniques: Expressing, Inhibition